Assay development
Cell transfection (transient and stable cell lines)
Crystallography
Electrophoretic mobility gel shift
Gene cloning and sequence analysis
Enzyme assay development for HTS
Flow cytometry
FRET
Florescent cell imaging (probe localization and intracellular trafficking)
High throughput/high content assay and critical path design and development
In silico drug-target interaction modeling
Isothermal Calorimetry
Macromolecular structural modeling
PAGE (1D, 2D) western blotting
PCR, multiplex PCR, real time PCR, RT-PCR
Protein and RNA expression
Size exclusion chromatography and ion exchange chromatography
Surface plasmon resonance
Thermal shift assay
Ultracentrifugation and sedimentation analysis

Chemical Libraries 25,000 diversity set library and assorted smaller libraries

• Mammalian
  • Set-up
    Janus Automated Liquid Handling System extended range deck
    Janus Automated Liquid Handling System mini
    BioTek MultiFlo FX Multimode Dispenser
  • Analysis
    Agilent BioTekCytation 1 Cell Imaging Multimode Reader
    Synergy 4 Multi-Mode Microplate Reader

LKB biochem Ultrospec II 4050 UV/visible Spectrophotometer
Vistavision VWR Upright Compound Microscope
Olympus IX81 and Hamamatsu Orac-C8484-05G02 camera
UVP ChemStudio Series

Two Forma seven foot BioSafety cabinets
Thermo Scientific Heracell 150i CO2 incubator
Forma Scientific CO2 water jacketed incubator Series II

Bacterial Based Assays

• Bacterial
  New Brunswick G24 Series Environmental Incubator Shaker
• Incubators/Shakers combinations
  Labline 4827J Orbit Environ-Shaker
  New Brunswick Scientific 25 Series Environmental Incubator Shaker
• Incubator/Heating Ovens/Water Baths
  VWR Pegasus 1545
  Labline Heat Block VWR
  VWR 1520 Heating Oven
  VWR 1420 Heating Oven/Vacuum
  VWR 1350G Forced Convection Oven
  ABS Incu-Cover
  Hierschler Ultrasound VialTweeter USS250V
  Brinkman RM20 Water Bath
  Isotemp 205 Water Bath
  VWR 1230 Water Bath
  VWR 1228 Water Bath

• Benchtop
  IEC HN-SII Centrifuge
  Beckman Coulter Alegra X-22R Centrifuge
  VWR Galaxy 14D Centrifuge
  Sorvall RT6000B Centrifuge
• Ultracentrifugation
  
  Sorvall RC-5B Super Centrifuge

  Beckman L8-M Ultracentrifuge

• Promega Express
  • Cabinet
  • Freezer
• Equipment
  • Electroporator
    BioRad MicroPulse Cell Electroporator
  • PCR
    PCRexpress Thermocycler
    StepOnePlus™ Real-Time PCR System
  • Gel Electrophoresis
    • Agarose
      BRL horizon 58 Horizontal Mini Gel Box
      H5-3023 Horizontal Gel Box
    • Polyacrylamide
      Polyacrylimide Gels/Transfers/Western Blots
  • Shakers/Tumblers
  • Chemistry Handling/Measurement
    Biological Safety Cabinet with vent to outside
    Terra Universal Series 100 Glove Box 1681-32E
    Brandel Plate Sealer
    Mettler Toledo A6104
    Mettler Toledo PB602-S/Fact
    SympHony SB70P
  • Storage
    -20°C Freezer
    -80°C Freezer
    4°C cold room
    Flame Cabinet
  • Spin blocks, heat blocks, hot plates, labquake mini, table top vortex, table top centrifuges, house deionized water, ultra pure water, house vacuum, house autoclave