Drug Discovery Services

Oyagen Capabilities

Technologies for Contracting Out

    1. Biochemistry, Biophysical, Cell Biology and Molecular Methods
      Assay development
      Cell transfection (transient and stable cell lines)
      Crystallography
      Electrophoretic mobility gel shift
      Gene cloning and sequence analysis
      Enzyme assay development for HTS
      Flow cytometry
      FRET
      Florescent cell imaging (probe localization and intracellular trafficking)
      High throughput/high content assay and critical path design and development
      In silico drug-target interaction modeling
      Isothermal Calorimetry
      Macromolecular structural modeling
      PAGE (1D, 2D) western blotting
      PCR, multiplex PCR, real time PCR, RT-PCR
      Protein and RNA expression
      Size exclusion chromatography and ion exchange chromatography
      Surface plasmon resonance
      Thermal shift assay
      Ultracentrifugation and sedimentation analysis

 

    1. High Through-put Facilities
      Chemical Libraries 25,000 diversity set library and assorted smaller libraries

 

    1. Optics/Mictroscopy/Spectroscopy
      LKB biochem Ultrospec II 4050 UV/visible Spectrophotometer
      Vistavision VWR Upright Compound Microscope
      Olympus IX81 and Hamamatsu Orac-C8484-05G02 camera
      UVP BioSpectrum Imaging System

 

    1. Mammalian Tissue Culture
      Two Forma seven foot BioSafety cabinets
      Thermo Scientific Heracell 150i CO2 incubator
      Forma Scientific CO2 water jacketed incubator Series II

 

    1. Microogranism-Based Assays
      Bacterial Based Assays

 

  1. Centrifugation
  2. Molecular Biology

Fee For Service

OyaGen offers method development and testing as a fee-for-service to academic and educational institutions and to industry. OyaGen staff will provide expert consulting services in assay development, biochemistry, structural biology, cell biology and virology for high throughput drug discovery and drug development as well as advice in preparing federal grants. OyaGen prides itself of the rigor and reproducibility of its science stemming from 75 years of collective experience that has been vetted through numerous peer reviewed publications and successful grant applications.

As a fee-for-service OyaGen will work with clients to design services that meet the needs of clients. These include but are not limited to:

(1) planning of critical path for assay development that spans the client’s goals
(2) Assistance in establishing and optimizing cell-based or defined in vitro assays
(3) Quantifying and validating drug effects on targets (EC50/EC90),
(4) Quantification of Proteins, RNA and DNA,
(5) Assessing cell proliferation and cell death,
(6) Determining subcellular localization of biomolecules
(7) pseudotyped HIV infectivity testing
(8) Understanding funding opportunity and consulting for grant writing
(9) Peer review of manuscripts prior to submission